INDEX（目次）に戻るATSUNORI ISHIDA,YASUNOBU YOSHIAKI,SHINJI MUROSAKI,
CHIHARU KUBO,YOSHIO HIDAKA AND KIKUO NOMOTO
Department of Immunology,Medical Institute of Bioregulation,
Kyushu University,Fukuoka 812, Laboratory of Germfree Life,
Research Institute for Disease Mechanism and Control,
Nagoya University School of Medicine,Nagoya 466
Department of Psychosomatic Medinine,Faculty of Medicine,
Kyushu University,and Food and Nutrition Division,Eisai Research
Laboratories,Eisai Co.,Ltd.,Saitama 367,Japan
ABSTRACT
Milk was obtained from nonimmunized cows and cows immunized with
a mixture of various human gut bacteria. Each milk was administered
orally to 2-month-old C57BL/6 mice at a dose of 150g/kg for either
6 or 16 months. The group had fewer enteric bacteria and a lower
concentration of the serum antibodies against enteric bacteria
compared with the control group at 8 and 18 months of age. Furthermore,
the study group at 18 months old had a higher redirected cytotoxicity
of intestinal intraepithelial lymphocytes, a higher proliferative
response of mesenteric lymph nodes cells against mitogenic or
alloantigenic stimulation and a greater ability of the spleen
cells to produce anti-sheep erythrocytes IgG antibody after systemic
immunization with sheep erythrocytes. A lower level of autoantibodies
was observed at 8 and 18 months of age in the study group compared
with the control group. These results suggests that the senescence
of the murine immune system may be delayed by consumption of milk
from immunized cows. J. Nutr. 122: 1875-1883, 1992.
1992 American Institute of Nutrition.
●日本語訳
免疫をしていないウシと、様々なヒト腸内細菌の混合物で免疫したウシのミル クを用意した。生後二ヶ月のC57BL/6マウスに、それぞれのミルクを一日当た
り150g/Kg、6カ月または16カ月間経口投与した。生後8カ月および18カ月の時 点で、対照群に比べて実験群のマウスは腸内細菌数が少なく、腸内細菌に対す
る血清抗体の濃度も低かった。そのうえ月齢18カ月の実験群は腸管上皮リンパ 球の強い間接細胞障害活性、腸管膜リンパ節細胞のマイトジェンや同種抗原に
対する高い増殖反応、そしてヒツジ赤血球による全身免疫後の脾細胞の非常に 高い抗ヒツジ赤血球IgG抗体産生能力を示した。8カ月および18カ月での自己抗
体値は、対照群に比べると実験群のほうが低かった。これらの結果はネズミの 免疫系の老化が、免疫ウシのミルクを飲用することで遅延できる可能性を示唆
している。