INDEX（目次）に戻るShinji Murosaki,1 Yasunobu Yoshikai,* Chiharu Kubo,1 Atsunori
Ishida,
Goro Matuzaki, Tomonobu Sato,** Katumi Endo** and Kikuno Nomoto
Department of Immunology, Medical Institute of Biology and
1Department of Psychosomatic Medicine, Faculty of Medicine, Kyushu
University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812
*Laboratory of Germfree Life, Research Institute for Disease Mechanism
and Control, Nagoya Unversity School of Medicine, 65 Tsurumai-Cho,
Showa-Ku, Nagoya 466; and
**Reaserch and Development Section, Takeda Food Products, Ltd,
3-20 Imoji, Itami, Hyogo 664, Japan
ABSTRACT
Autoimmune disease-prone (NZB×NZW)F1 (B/W) female mice are a model
of human lupus erythematosus. Immune milk, obtained from cows
immunized with various intestinal bacterial antigens, was given
to B/W mice as a component of diets beginning at 8 weeks of age.
Diets were fed ad libitum or restricted to 60% of ad libitum energy
intake. Controls were fed commercial skim milk. In B/W mice fed
ad libitum, the titers of anti- single-stranded DNA antibodies
were significantly lower in immune milk-fed mice at 4 and 6 months
of age. Onset of proteinuria was delayed and life span was significantly
prolonged by immune milk feeding. Surface phenotypes of the T
cells and levels of the responsiveness of lymphocytes to mitogens
were not changed by immune milk feeding. The B/W mice restricted
to 60% of ad libitum energy intake, which preserved immune responsiveness,
had not developed proteinuria by 14 months of age, irrespective
of immune milk feeding or control milk feeding. However, at 10
months of age, the level of plasma antibodies against intestinal
bacteria was significantly higher in energy-restricted mice fed
control milk than in those fed immune milk or in mice fed ad libitum.
J. Nutr. 121: 1860-1868, 1991.
1991 American Institute of Nutrition.
●日本語訳
自己免疫疾患傾向のある（NZB×NZW）F1（B/W）雌マウスは、ヒトエリトマトー デスの疾患モデルである。多種の腸内細菌抗原で免疫化したウシから得られた
免疫ミルクを飼料に混ぜて、8週齢から（B/W）マウスに与えた。飼料は自由摂 取させるか、自由摂取時の60％となるように制限した。対照群は市販のスキム
ミルクとした。自由摂取させたマウスでは、免疫ミルク混餌飼料を与えたほう が4カ月齢及び6カ月齢における抗一本鎖DNA抗体の力価が有意に低かった。免
疫ミルクを与えたマウスでは、蛋白尿の開始が遅れ、寿命が有意に伸びた。Ｔ 細胞の表面型及びマイトジェンへの白血球の反応性は、免疫ミルクを与えても
変わらなかった。エネルギー摂取を60％に制限したマウスでは、免疫ミルク群、 対照ミルク群に関わらず、免疫反応性を維持しながらも、14カ月まで蛋白尿と
ならなかった。しかし、10カ月齢になると、対照ミルクを与えてエネルギー摂 取を制限したマウスのほうが、免疫ミルクを与えたり自由摂取させたマウスに
比べて、腸内細菌に対する血漿抗体レベルが有意に高くなった。